小鼠膜性肾病肾脏中MMP-2的表达及意义

EXPRESSION OF MMP-2 PROTEIN IN KIDNEY OF MICE WITH MEMBRANOUS NEPHROPATHY

目的:探讨肾脏MMP-2的表达与膜性肾病发病机制间的关系。方法:把小鼠随机分为膜性肾病模型组和对照组,用免疫组织化学(SABC法)检测肾脏组织中MMP-2的表达,用图像分析系统检测基底膜厚度。结果:模型组和对照组肾脏中均有MMP-2表达,且主要表达在肾小管上皮细胞胞浆中。模型组肾组织中MMP-2表达明显强于对照组(P<0·01),且模型组中MMP-2表达与GBM厚度呈正相关(0·01<P<0·05),对照组MMP-2表达与GBM无相关性(P>0·05)。结论:在MN过程中,肾脏中MMP-2参与了GBM增厚形成的调节机制,提示在MN发病中,细胞外基质(ECM)聚集的同时,可能通过反馈调节机制促使肾脏组织中MMP-2表达上调,从而可能在一定程度上减少ECM的过度堆积。

Objective： To research the relativity between mechanism and MMP- 2 protein expression in mice with membranous nephropathy.Methods： Membranous nephropathy mice animals model were used, and the MMP- 2 protein expression in kidney was assessed by immunohistochemistry and the thicknesses of GBM and TBM were measured. Then the statistical methods were used to compare and analyse the data of MN group and control group. Results： MMP - 2 protein were expressed mainly in renal tubule of MN group and control group. The level of MMP- 2 protein in MN group increased remarkably comparing with control group. In MN group, the expressions of MMP- 2 protein paralleled with the thickness of GBM. And in control group, the expression of MMP - 2 protein were not relative to the thickness of GBM; Conclusion： In MN, the abnormal expressions of MMP - 2 protein were relative to the mechanism of MN, and MMP- 2 may play an important role in preventing the developing of MN.