葡萄糖酸锌和硫酸镁拮抗二氧化硅细胞毒性作用的研究

EFFECT OF ZINC GLUCONATE AND MAGNESIUM SULFATE ON ANTAGONISM TO THE TOXICITY OF SILICA IN ALVEOLAR MACROPHAGES

目的:观察葡萄糖酸锌与硫酸镁单独及联合作用对二氧化硅致肺泡巨噬细胞丙二醛、超氧化物歧化酶及谷胱甘肽过氧化物酶水平变化的影响,寻找二者的最佳剂量组合。方法:采用大鼠肺灌洗法纯化获得肺泡巨噬细胞(1×109个/L),同时加入二氧化硅及不同浓度的葡萄糖酸锌溶液、硫酸镁溶液、葡萄糖酸锌与硫酸镁溶液,37℃,5%CO2培养箱培养18h后,检测丙二醛、超氧化物歧化酶及谷胱甘肽过氧化物酶含量。结果:葡萄糖酸锌与硫酸镁单独作用,以及两者联合使用都具有拮抗二氧化硅细胞毒性作用。且以1·5mg/L葡萄糖酸锌的与2·0μmol/L硫酸镁联合作用效果最好。结论:葡萄糖酸锌与硫酸镁单独及二者联合应用在体外可有效拮抗二氧化硅粉尘的细胞毒性作用,降低肺泡巨噬细胞的丙二醛含量,升高巨噬细胞的超氧化物歧化酶及谷胱甘肽过氧化物酶的活力,二者合用效果更佳。

Objective： To investigate the effect of zinc gluconate and magnesium sulfate used either solely or together on antagonism to the toxicity of silica in alveolar macrophages. Methods; Alveolar macrophages were cultured with silica, at the same time zinc gluconate, magnesium sulfate and zinc gluconate together with magnesium sulfate were added respectively. Malondialdehyde （MDA）, Superoxide dismutase （,SOD） and Glutathine Peroxidase （GSH - Px） in alveolar macrophage were measured. Results： Zinc gluconate, magnesium sulfate and zinc gluconate together with magnesium sulfate have effects of contradict the toxicity of silica in alveolar macrophages. The most suitable combination of the two substances should be 1.5 mg/L of zinc luconate together with 2.0 μmol/L of magnesium sulfate. Conclusion： Zinc gluconate and magnesium sulfate used both solely or together could reduce the levels of MDA and increase SOD and GSH- Px in alveolar macrophages exposed to silica.