蜂胶复方消毒剂杀菌效果与毒性的试验观察

EXPERIMENTAL OBSERVATION ON GERMICIDAL EFFICACY AND TOXICITY OF COMPOUND DISINFECTANT OF BEE GLUE

目的：了解蜂胶复方消毒剂的杀菌效果与毒性。方法：采用载体定量与动物试验法，分别观察了其杀菌效果与毒性。结果：其原液按1：6加水稀释液对布片上大肠杆菌、金黄色葡萄球菌作用15min，杀灭率为100％；按1：2加水稀释液对布片上白色念珠菌作用20min，杀灭率为99．96％～99．98％。杀菌效果因有机物存在或该剂存放于54℃下14d而有所下降。其原液对小鼠口服LD50〉5000mg／kg，对家兔皮肤刺激反应积分为0，属实际无毒、无刺激物质。结论：蜂胶复方消毒剂是一种具有较好杀菌效果、使用安全的新型消毒剂。

Objective： In order to know germicidal efficacy and toxicity of compound disinfectant of bee glue. Methods： The carrier quantitative germicidal test and animal test were used to observe its germicidal effcacy and toxicity. Results： The result showed that dilute solution formed by mixing its stock solution with water at a ratio of 1：6 with a 15 minute contact time could kill 100% of Escherichia coli and staphylo coccus attreus on cloth strip and the dilute solution formed by adding water at a ratio of 1：2 with a 20 miunte contact time could kill 99.96 % -99.98 % of Candida albicans on cloth strip. The germicidal efficacy decreased due to presence of organic substances or after storing at 54 ℃ for 14 days. The oral LD50 of its stock solution in mice was 〉5 000 mg/ kg and the integrated score of skin irritative reaction in rabbits was zero, so it belongs to actually non- toxic and non-irritative substance. Conclusion： Compound disinfectant is a kind of original disinfectant which has better germicidal efficacy and could be safely used.