虎奶菇菌核蛋白多糖的分离纯化及化学结构分析

Isolation, Purification and Chemical Structure of a Pleurotus tuber-regium Proteoglycan

虎奶菇菌核干品经水提、醇沉、Sevage法除蛋白、透析得纯蛋白多糖。分析结果表明,该纯蛋白多糖分子质量为39810,糖含量为61.7%,氮含量为38%,由葡萄糖、半乳糖等单糖组成,糖基连接键以β-1,4为主,还有β-1,3连接键。

A proteoglycan with a molecular mass of 39810 as determined by gel filtration, was obtained from dry, powdered sclerotia of Pleurotus tuber-regium following extraction with water, ethanol precipitation, protein removal by the Sevage method, dialysis, and Sephadex G-75 chromatography. Chemical analyses revealed the material to consist of 61.7% total sugar （calculated as glucose） and 38% nitrogen, and gas chromatography of acid-hydrolysis products indicated that the major monosaccharide components were glucose and galactose. Smith degradation data suggested that the main glycosidic linkage in the proteoglycan was β-1,4, and that β-1,3 linkages may also be present.