存在于奶牛Ig重链和多种动物Ig分子的两种相同抗原结合位点的研究

The Study of Two Same Antigen Binding Sites at Bovine Ig Heavy Chain and at Ig一molecules of Some Species

在应用ＥＩＪＳＡ鉴定７株抗奶牛ＩｇＭ、ＩｇＡ、ＩｇＧ＿１、ＩｇＧ＿２和Ｉｇ一轻链的单克隆抗体（ｍＡｂ）反应性中发现，这些ｍＡＢ均是特异地与相应ｌｇ分子中的轻链或重链的抗原决定簇结合，用夹心法和抑制法均证明了这点。还发现，在奶牛ＩｇＡ的ａ一链和ＩｇＭ的μ一链上有相同的结合位点。与其他动物血清和经盐析Ｉｇ的反应结果表明，其中有一株抗Ｉｇ一Ｌ（轻链）的ｍＡｂ能与狗和海狗Ｉｇ较好地结合，与马和骆驼的ｌｇ亲和力轻小，但不能与猫、豚鼠、大白鼠、猪和家兔的Ｉｇ结合。这表明，不同种的动物Ｉｇ存在着共同抗原决定簇。

The results of identification of some kinds of monoclonal antibodies(mAb)against bovine IgM,IgA,IgG1,IgG2 and light chain(L一chain) by ELISA showed thatthese mAbs would specifically bind antigenic determinant at light chain or heavy chains of lgmolecules by Sandwich’and inhibitive ELISA, and that there mihgt be common antigenbinding site at α-chain(IgA)and μ-chain(IgM) of bovine lg molecules.In addition,one ofmAbs anti L一chain cou.ld bind lg molecule of sonie kinds of animals:dog,seadog and in aweak degree horse and camel from serums and Ig purified by salting out,but not of cat,seaswine,rat,swine and rabbit.All these results suggest that there might be common antigenrecognition site at the Ig molecule from different species.