摘要
本文报道了应用聚丙烯酰胺凝胶垂直板电泳(PAGE)分析研究分布于稻田的三种不同体色泥鳅在心肌、骨骼肌、肝脏、眼、血清七种组织器官中LDH同工酶的表达特征。电泳图谱表明,在这些组织中,主要存在LDH2和LDH3,其他同工酶的活性较低,甚至没有。定量分析进一步表明LDH同工酶的组织分布和活力均具有明显的组织特异性:在脑组织LDH2活性最强,其它组织如血清、眼、卵巢、肝脏、心肌等组织中LDH3活性最强。此外,在肝脏中存在由Ldh一C编码的C带。
The electrophoretic patterns of LDH isoenzymes in serum,eye,brain,o-vary,liver,skeletal muscle and cardiac muscle from adult M,anguillicaudatus expressed bythree dinds of body colours and distributed over rice field were studied by means of polyacry-lamide gel vertical electropheresis,The results indicated taht in these tissues there were twobands,namely LDH2 and LDH3,the activity of other LDH isoenzymes expressed low,evenno.The quantitative analysis exhibited a tissue specific expression in tissue distribution andenzyme-activity:the strongest activity of the LDH isoenzyme were LDH2 in brain,LDH3in other tissues,namely in serum,eye,ovary,liver and cardiac muscle.In addition,in liverthere was a C-band codied by Ldh-C.
出处
《安徽农业大学学报》
CAS
CSCD
1996年第2期155-159,共5页
Journal of Anhui Agricultural University
关键词
泥鳅
LDH同功酶
稻田
Migurnus anguillicaudatus LDH isoenzyme Tissue-specific