油菜花药中酯酶和柱头中角质酶的提纯

Purification of Esterase in Anthers and Cutinase in Stigmas from Brassica campestris

油菜（ＢｒａｓｓｉｃａｃａｍｐｅｓｔｒｉｓｃｖＴ１５）授粉时期花药和柱头总蛋白质提取液经ＳＤＳ聚丙烯酰胺凝胶电泳分离，花药中酯酶用α－乙酸萘酯和β－乙酸萘酯染色，柱头中角质酶用４－Ｍｅｔｈｙｌｕｍｂｅｌｌｉｆｅｒｕｌｂｕｔｙｒａｔｅ染色。切下的酶带纯化后，经ＳＤＳ聚丙烯酰胺凝胶电泳检测，酯酶分子量约为３１ＫＤ，角质酶分子量约为３７ＫＤ。

Protein extracts from anthers and stigmas of Brassica campestris at pollina-tion stage were isolated on SDS-pAGE,Esterase from anthers was stained with α-naph-thvl-acetate and β-naphthyl-acetate.Cutinase from stigmas was stained with 4-methy-lumbellifervl butvrate.After esterase and cutinase bands were excised from PAGE and puri-fied,they were checked on SDS-pAGE.Molecular weight of esterase is 31KD,cutinase37KD;