环境汞污染粮食暴露对大鼠脑自由基的诱导及共存硒的影响

Induction of Free Radicals After Dietary Exposure by Mercury Contaminated Rice and Protective Effect of Coexisting Selenium

采用电子顺磁自旋共振(ESR)技术,研究实地环境汞污染粮食暴露不同时间后在大鼠脑中引发的自由基及脂质过氧化损伤情况,探讨实地环境汞污染氧化损伤的微观致毒机理.并对粮食中共存的硒元素作用进行分析.结果表明,环境汞污染粮食暴露7d后,大鼠脑中自由基相对强度及脂质过氧化产物MDA含量均出现显著升高(p<0.05).ESR图谱分析表明,环境汞污染粮食暴露诱发大鼠脑中产生烷基自由基(R.)及羟基自由基(.OH).自由基及MDA水平在暴露前期(<90d)相对稳定,长暴露90d后,自由基及脂质过氧化产物MDA水平有大幅升高(83%,100%).大鼠脑中汞、硒元素含量7d暴露后均出现显著上升,2元素间有较强相关性,暴露前期大鼠脑中自由基水平相对稳定应与硒的清除作用有关.

Sprague-Dawley rats were reared by environmental mercury contaminated rice to survey the potential health risk of Wanshan mercury mining area. Electron spin resonance （ESR） was introduced to detect the species and the intensities of free radicals, using spin trap 5,5-dimethyl-1-pyrroline-N-oxide （DMPO）. The results showed that the mercury-contaminated rice significantly increased the levels of free radicals and MI）A in rat brain at 7 days （p 〈 0.05）. ESR spectrums showed that the principal spin adducts resulted from the trapping of alkyl free radical （αH = 22.7 × 10^-4T± 1.6 × 10^-4T, αN = 15.5× 10^-4T ± 0.5 × 10^-4T）, and hydroxyl radical. Levels of free radicals and MDA increased slowly until after 90-day exposure period （83%, 100% ）. Element correlation analysis showed high correlations of mercury and selenium in the brain of rat fed with Wanshan rice, suggesting that the coexisting selenium in rice exhibited antagonistic effects on both mercury accumulation and toxicity. The slight increases of free radicals in rat brain at 7, 20 and 30-day exposure periods should be related with the scavenger effect of Se.