摘要
利用改进的提取沙拐枣Calligonum mongolicum DNA的CTAB方法，从沙拐枣当年鲜根提取总DNA，以琼脂糖凝胶电泳和紫外分光光度计检测，并进行PCR扩增试验。结果显示，所提取的DNA片段大小在20kb以上，OD260／OD280比值为1．880～1．900，OD260/OD230比值为2．000～2．040，DNA的浓度0．380～0．470 μg／mL，DNA纯度较高，可直接用于随机扩增的DNA多态性（RAPD）标记，条带清晰、迁移率低而整齐。
DNA was extracted from fresh root of Calligonum mongolicum using an improved method of CTAB,and examined by agarose gel electrophoresis, UV-spectrophotometer and PCR. The results showed that DNA fragment extracted by this method was larger than 20 kb,and ratios of OD260/OD280 and OD260/OD230 were 1. 880- 1. 900 and 2. 000- 2. 040, respectively. The concentrations of DNA were 0. 380-0. 470 μg/mL. The higher purity DNA can be directly used in RAPD with clear bands and low transferring rate.
出处
《草业科学》
CAS
CSCD
2006年第10期28-31,共4页
Pratacultural Science
基金
甘肃省自然科学基金(ZS031-A25-051-D)
