中国野生鸭茅遗传多样性的ISSR研究

Genetic diversity of wild Dactylis glomerata in China detected by inter-simplesequence repeat(ISSR) molecular markers

以宝兴鸭茅和国外安巴鸭茅品种为对照．用1SSR分子标记技术对四川、重庆、云南、贵州、新疆等地的32份野生鸭茅材料进行遗传多样性研究。试验筛选出引物12个．共扩增出多态性带88条，平均每个引物扩增的多态带数为7．3条．多态性条带比率（PPB）为83．18％．材料间遗传相似系数为0．6920～0．9260。这说明我国鸭茅具有较丰富的遗传多样性。根据研究结果进行了聚类分析和主成分分析．可将32份中国野生鸭茅材料分为五大类，同一地区的鸭茅品种（系）基本聚在同一类．呈现出一定的地域性分布规律。并对我国鸭茅种质资源的收集保存提出建议。

In a comparison of Dactylis glomerata cv. Baoxing with D. glomerata cv. Amba, Inter-simple Sequence Repeat （ISSR） molecular markers were used to detect the genetic diversity among 30 specimens of D.glomerata. Collected from Siehuan, Chongqing, Yunnan, Guizhou, and Xinjiang provinces. The twelve primers selected for use and produced a total of eighty-eight polymorphie bands, an average of 7.3 bands from each pair of ISSR primers. The average percentage of polymorphic bands was 83. 8%, and the range of GS was 0. 692 0-0. 926 0, which indicated a rich genetic diversity of D. glomerata in China. Based on cluster and principal component analyses of the genetic characteristics, the wild D. glomerata could be divided into 5 groups according to the nearest phylogenetic relationship. In most cases materials from the same region were in the same group thus showing a geographical distribution of genetic diversity of the tested materials. Advice is given on the collection and conservation of germplasm resources in wild D. glomerata populations.