胡枝子属野生居群遗传多样性的等位酶分析

Allozyme analysis of genetic diversity of wild Lespedeza

利用采自北京及其周边地区14个野生胡枝子属植物居群的种子培育出的幼叶材料,采用聚丙烯酰胺凝胶电泳技术,用改进的Tris-HCl复杂提取缓冲液(0.1%巯基乙醇和20%PVP 40 000)开展等位酶分析,成功地获得了7种酶的清晰谱带,共获得18个等位酶位点。对这18个位点等位基因的统计分析表明,野生胡枝子属群体多态位点的百分数为P=54.62%,等位基因平均数为A=1.743 7,平均每个位点等位基因的有效数目Ae=1.493 9,实际杂合度为Ho=0.384 5,预期杂合度为He=0.259 8,Shannon’s information Index的平均值I=0.378 2;杂合性基因多样度比率FST的平均值为0.446 7;居群每代迁移数Nm的平均值为0.309 6;以上数据说明胡枝子属植物各居群间存在较高的遗传分化程度。以Nei的遗传距离得到的聚类分析结果与形态学性状获得的结果基本一致。

Genetic diversity was estimated by vertical slab polyacrylamide gel electrophoresis in fourteen wild Lespedeza populations from Beijing and surrounding regions. With improved complexity Tris-HCl extraction buffer, seven enzymes, including 18 loci were assessed for allozyme genetic diversity. The percentage of polymorphic loci （P）, average allele number （A）, mean observed heterozygosity per locus （Ho）, expected heterozygosity per locus （He）, Shannon＇s information Index （I）, ratios of gene diversities of heterozygosities （FST） and gene flow （Nm） of 14 Lespedeza populations were 54.62%, 1. 743 7, 0. 384 5, 0. 259 8, 0. 378 2,0~ 447 6 and 0. 309 6, respectively. Results of allozyme cluster analysis based on Nei＇s genetic distance agreed with the results of morphological cluster analysis.