赤霉素对小麦幼苗乙醇酸氧化酶活性的影响

Effect of GA 3 on the Activity of Glycolate Oxidase in Wheat Seedlings

研究结果表明，３００～１０００ｍｇ／Ｌ的赤霉素处理小麦幼苗后，小麦体内乙醇酸氧化酶活性提高１８．７５％～４３．７５％，正相关系数ｒ＝０．９６５５；５００ｍｇ／Ｌ赤霉素处理后，乙醇酸氧化酶活性变化与处理时间呈正相关，直线回归方程为ｙ＝０．０７９６＋０．００１２３ｘ。３００～１０００ｍｇ／Ｌ的赤霉素处理使小麦蛋白质含量升高１５．１６％～３７．７４％，正相关系数ｒ＝０．９７１４；赤霉素对离体乙醇酸氧化酶活性无影响。

The result of this investigation indicated that the treatment with 300￣ 1 000 mg/L gibberellin A 3 (GA 3) raised the activity of glycolate oxidase in wheat seedlings by 18.75% ￣43.75%, the (GA 3) correlation coefficient is r =0.9655. When the wheat seedlings were treated with 500 mg/L GA 3, the relation between the activity of glycolate oxidase and the time after treatment was positively correlative. The linear regression equation of that was: y=0.0796+0.00123x. Treatment with 300￣1 000 mg/L GA 3 raised the content of protein by 15.16%￣37.74%, the correlation coefficient was r =0.9714. The GA 3 had no effect on the activity of glycolate oxidase in vitro. The promotion effects of GA 3 on the protein biosynthesis and the activity of glycolate oxidase could be inhibited by the treatment with cycloheximide. The possible action ways of GA 3 affecting the activity of glycolate oxidase were also discussed in this paper.