消栓通脉颗粒剂对深静脉血栓形成模型大鼠血浆t-PA PAI的影响

Effect of Xiao Shuan Tong Mai Granules on Plasma Tissue-type Plasminogen Activator and Plasminogen Activator Inhibitor after Deep Venous Thrombosis in Rats

目的：探讨消栓通脉颗粒剂对深静脉血栓形成模型大鼠血浆t—PA、PAI的影响。方法：将160只Wistar大鼠随机分为6组：空白对照组、模型对照组、消栓通脉高、中、低剂量组和通塞脉对照组。采用下腔静脉结扎法制备血栓模型，检测血栓形成后不同时期血浆t—PA、PAI活性的变化和观察扫描电镜下内皮层的改变。结果：消栓通脉颗粒剂具有显著提高t—PA活性，降低PAI活性作用，其高、中剂量组提高t—PA活性的作用优于模型和通塞脉对照组，有显著性差异（P〈0．01—0．05，P〈0．01—0．05）；抑制PAI活性的作用，消栓通脉各组与模型对照组比较，均有非常显著性差异（P〈0．01），且高剂量组抑制PAI活性的作用明显优于通塞脉对照组（P〈0．05）。电镜观察消栓通脉颗粒剂组内皮损伤明显减轻。结论：消栓通脉颗粒剂治疗深静脉血栓形成的机理，与保护血管内皮细胞，促进纤溶功能作用有关。

Objective ：To study the effect of Xiao Shuan Tong Mai Granules （XSTM） on plasmatissue -type plasminogen activat or（ t - PA） and plasminogen activat or inhibitor （PAl） after deep venous thrombosis in Rats. Methods ： 160 Wistar rats were randomly divided into 6 groups ： normal group, thrombosis group, high - dose XSTM group, media - dose XSTM group, low - dose XSTM group ,Tong Sai Mai group. Venous thrombosis was induced by inferior vena caval（IVC） occlusion by ligature just below the level of the renal veins. Plasma t - PA and PAl were detected on the 1st ,3rd,6th days after using different drug and inferior vena eaval was observed by scanning electricity mirror. Results ： Plasma t - PA was low and PAl maintained high level after venous thrombosis. The XSTM can increase plasma t - PA and decrease PAl. Significant difference were found between XSTM groups and thrombosis group, Tong Sai Mai group on t - PA and PAl. Conclusion ： The XSTM can increase plasma t - PA, decrease PAl and protect the vein structure.