蚤休和半边莲对大鼠脑出血后血浆细胞间黏附分子-1肿瘤坏死因子-α的影响

Effect of Rhizoma Paridis and Herba Lobeliae Radicantis on Changes of Superoxide Intercellular Adhesion Molecular-1(ICAM-1)and Tumor Necrosis Factor-α(TNF-α) Content in Plasmaafter Intracerebral Hemorrhage in Rats

目的:观察清热解毒药物蚤休、半边莲大鼠脑出血后血浆细胞间黏附分子-1(ICAM-1)、肿瘤坏死因子(TNF-α)的影响,以探讨蚤休、半边莲治疗脑出血的作用机制。方法:实验大鼠随机分为假手术1组、2组,模型1组、2组,中药1组、2组,采用胶原酶Ⅶ诱导大鼠脑出血模型,术后2h,中药1组、2组给予蚤休、半边莲水煎剂灌胃,另4组给予等量生理盐水,连续用药(1次/日),分别在3天、7天后取血,应用酶联免疫吸附法和放射免疫分析法测定血浆ICAM-1、TNF-α含量。结果:与假手术组比较,模型组血浆ICAM-1和TNF-α含量均明显升高(P<0.01),与模型组比较,中药组血浆ICAM-1和TNF-α含量均明显下降(P<0.01,P<0.05)。结论:中药蚤休、半边莲对脑出血后神经细胞损伤具有保护作用,其机制可能与减少ICAM-1和TNF-α的产生,抑制炎症反应有关。

Objective： To observe Rhizoma Paridis and Herba Lobeliae Radicantis on changes of Superoxide Intercellular Adhesion Molecular- 1 （ICAM -1 ）and Tumor Necrosis Factor- α（ TNF -α） content in plasma after intracerebral hemorrhage in rats and make a study on mechanism of treatment of it. Methods ： Rats were randomly divided into six groups ： sham - operative groupl and 2, model groupl and 2, Herbs groupl and 2. CollagenaseⅦ was injected into caudate nucleus to establish rat model of intracerebral hemorrhage. After 2 hours, Rhizoma Paridis and Herba Lobeliae Radicantis decoction was given by gastrogavage in Herbs groupl and 2,saline was given in the other 4 groups. This kind of management was consecutively done,once a day for 3 and 7 days. Fluorospectrophotometry and radioimmunoassay were used to measure the content of ICAM - 1 ,TNF - α in plasma. Results ： Compared with sham-operative group, ICAM -1 and TNF-α content in plasma were obviously higher（ P 〈0.01 ） in model group, Compared with model group, ICAM - 1 and TNF - α content in plasma were obviously lower（ P 〈 0. 01 ,P 〈 0.05 ） in Herbs group. Conclusion ： Rhizoma Paridis and Herba Lobeliae Radicantis had an evident protective effect on injured neuron after intracerebral hemorrhage in rats, which may be related to decreasing ICAM - 1 ,TNF - α content in plasma and inhabiting inflammatory reaction.