反相高效液相色谱法测定板蓝根及制剂中腺苷的含量

Determination of Adenosine Content in Banlangen and Banlangen Granule by HPLC

目的：建立一种用反相高效液相色谱法测定板蓝根及（制剂）板蓝根颗粒剂中腺苷的含量。方法：以Hypensil ODS C18（5μm，4．6mm×200mm）为色谱柱，甲醇-0．05mol／L磷酸二氢钾（15：85），为流动相；检测波长为：UV-260nm，流速：1．0mL／min，柱温：25℃，外标法定量。结果：腺苷在4．16～35、4μg／mL，范围内呈现出良好的线性关系，（r=0．9999）；方法平均回收率为99．67％（n=9），相对标准偏差RSD为1．3％。结论：该方法简便、快速，测定结果准确可靠，可用于板蓝根及板蓝根颗粒的质量控制。

Objective： To develop a determination method for adenosine content in Banlangen and （preparation） Banlangen granule by opposite HPLC. Methods： Eluted with carbinol -0.05mol/L potassium dihydrogen phosphate （ 15：85 ） in a flow rate of 1.0 mL/min , adenosine was analyzed under the wavelength of 260 nm in Hypensil ODS C18 column （5 μm ,4.6mm × 200mm）. The column temperature was 25. Fix quantify by external standard method. Results： Adenosine presented wellordered linear dependence in the range of 4.16 - 35.4 μg/mL （ r = 0.9999 ）, the recovery of the assay was 99.67 % （ n = 9 ）, the RSD was 1.3 %. Conclusion ： The method ,accurate and reproducible , was suitable for the quality assessment of Banlangen and Banlangen granule.