牙科手机传播乙型肝炎可能性的动物实验研究

Infective Capability of Dental Bur Contaminated by Sera Containing DHBV: An in vivo Experimental Study

目的用鸭乙型肝炎病毒(DHBV)血清污染牙科手机,观察污染剂量在斑点杂交方法检不出的情况下,是否具有对动物的感染性。方法用强阳性鸭肝炎病毒污染的牙科手机,将手机洗脱液一部分运用斑点杂交法对残留病毒DHBV-DNA进行检测作为体外实验;另一部分转染1d龄北京鸭,10d后取血检测DHBV-DNA复制情况作为体内实验。结果体外实验检测结果均呈现阴性,OD值最大为0.422,而体内实验的检测结果发现所有鸭子体内均有不同程度的DHBV-DNA复制,OD值最大达到0.861。结论即使DHBV污染的牙科手机在斑点杂交检测为阴性结果的情况下,也不能排除传播肝炎病毒的可能。

OBJECTIVE To evaluate the in vivo infective capability of dental bur contaminated by sera containing DHBV at an undetectable dose checked by dot blot hybridization. METHODS The dental burs were contaminated by DHBV-posiuve duck sera. The DHBV levels of eluting solution from contaminated dental burs were detected by dot blot hybridization. An in vivo experimental study was made by injecting the same eluting solution into the veins of one-day Beijing ducks. After ten days, the levels of DHBV in the duck sera were detected by dot blot hybridization. RESULTS In the in vitro test, the optical densities of dot blot hybridization of eluting solution from contaminated dental burs were not higher than those of negative controls. But in the in vivo test, the levels of DHBV in the duck sera of the experimental groups were found to be significantly higher than the negative control groups. CONCLUSIONS Although the levels of DHBV of eluting solution from contaminated dental burs are at an undetectable dose checked by dot blot hybridization, the dental burs still have the possibility of transmitting the virus.