儿童抽动-秽语综合征HCMV pp65抗原检测的临床意义

The Clinical Significance of the Human Cytomegalovirus pp65 Antigen Test for Diagnosis of Tourette's Syndrome in Children

目的探讨儿童巨细胞病毒(Hum an cytom egalov irus,HCMV)活动性感染与抽动-秽语综合征(T ourette’s sym-drom e,TS)的关系。方法离心分离64例抽动-秽语综合征患儿和40例正常儿童外周血单个核细胞(PBM C s)和血浆,分别用免疫荧光法和定量PCR检测HCMV pp65抗原和HCMV DNA,并比较2种方法的一致性。结果64例TS患儿HCMV pp65抗原有14例阳性,阳性率21.9%,40例正常儿童HCMV pp65抗原有1例阳性,阳性率2.5%,2组儿童HCMV感染率有显著性差异(2χ=7.49,P<0.01)。免疫荧光法和实时定量PCR有较好的一致性(89.1%)。结论抽动-秽语综合征儿童HCMV感染率显著高于正常儿童,HCMV活动性感染可能诱发抽动-秽语综合征。

Objective To investigate the relation between Tourette＇s syndrome （TS） and active human cytomegalovirus （HCMV） infection. Methods 64 blood ,specimens from the children with TS and 40 blood specimens from the normal children were collected. The peripheral blood mononuclear cells （PBMCs） were separated from plasma,and the former was used for the HCMV pp65 antigen test by immune fluorescent assay （IFA） and HCMV DNA was quantitative analysis by quantitative polymerase chain reaction （PCR） in parallel comparison with the HCMV pp65 antigen test. Results 21.9% TS children were positive to HCMV pp65 antigen and only 2.5% normal children were positive. The correspondence of the HCMV pp65 test and quantitative PCR was 89. 1%. Conclusion The rate of HCMV infection in TS children markedly high than that of normal children, it suggested that HCMV infection perhaps be one of induced factor of TS in children.