摘要
以马铃薯栽培品种甘农薯1号的试管苗为材料，进行了茎尖超低温保存技术的研究，结果表明，预培养3～4d，用玻璃化溶液PVS2室温下处理20～30min，直接投入液氮中冷冻保存，然后接种在MS添加2．50mg／LKT、1．25mg／LIAA和1．25mg／L6-BA的培养基上，经过恢复培养，茎尖成活率可达42．8%。
Shoot tips of plantlet in vitro of potato cultivar Gannongshu 1 as donors, cryopreservation of shoot tips was studied in the experiment. The excised shoot-tips, were precultured 3-4 d and treated by vitrification solution PVS2 for 20- 30 rain under room temperature, plunged into fluid nitrogen and stored for a certain time then transferred onto MS medium supplemented with 2. 5 mg/L KT, 1.25 mg/L IAA and 1.25 mg/L 6-BA, survival rate of shoot tips of potato could reached 42.8%.
出处
《甘肃农业科技》
2006年第10期3-5,共3页
Gansu Agricultural Science and Technology
关键词
马铃薯
茎尖
超低温保存
Potato
Shoot-tip
Cryopreservation
