摘要
本文基于维生素B1(VB1)、维生素C(VC)对鲁米诺(Luminol)和铁氰化钾(K3[Fe(CN)6])化学发光反应的增敏作用原理,建立了反相高效液相色谱(RP-HPLC)分离、柱后化学发光检测VB1与VC的新方法。当在鲁米诺中加入KBr时可以大大增敏该反应。本法已成功运用于蜂蜜和复合维生素片中VB1与VC的测定。
A novel high performance liquid chromatograph chromatography (HPLC)-Chemiluminescence (CL) detection method for simultaneous determination of VB1 and VC based on the chemiluminescence reaction of thiamin (VB1) and ascorbic acid (VC) with Luminol-FeK3(CN)6-KBr system was developed. The proposed method has been applied to the determination of VB1 and VC in a pharmaceutical and honey preparation.
出处
《分析科学学报》
CAS
CSCD
2006年第5期533-536,共4页
Journal of Analytical Science
基金
重庆市自然科学基金(CSTC-2004BB7087)
关键词
高效液相色谱
化学发光
维生素B1
维生素C
High performance liquid chromatography
Chemiluminescence
Thiamin
Ascorbic acid