高效液相色谱-串联质谱法同时测定水溶性迷迭香提取物中迷迭香酸、阿魏酸和咖啡酸的含量

Simultaneous Determination of Rosmarinic Acid,Caffeic Acid and Ferulic Acid in Water-soluble Extract of Rosemary by LC/MS/MS

本文建立了一种同时快速测定水溶性迷迭香提取物中迷迭香酸、阿魏酸和咖啡酸含量的高效液相色谱-串联质谱（HPLC／MS／MS）分析方法。以甲醇和0．1％乙酸铵溶液为HPLC的流动相；MS／MS使用多反应监测（MRM）扫描方式，在3min内可完成迷迭香酸、阿魏酸和咖啡酸三种化合物的分离分析。上述三种分析物在5～500ng／mL范围内线性良好（r〉0．999）；检出限均低于5．0ng／mL。本法操作简便、分析速度快、灵敏度高，可用于水溶性迷迭香提取物中迷迭香酸、阿魏酸和咖啡酸含量的测定。

A rapid LC/MS/MS method was developed for the simultaneous determination of rosmarinic acid,caffeic acid and ferulic acid. The mobile phase of HPLC was methanol-0.1% ammonium acetate aqueous solution. As to MS/MS,multi-reaction monitoring （MRM） scan was employed,and a complete analysis can finished in three minutes. The calibration curves for three analytes were linear in the range from 5 ng·mL^-1 to 500 ng·mL^-1. The developed method was rapid,accurate,robust and convenient, and it was applied to the measurement of rosmarinic acid, caffeic acid and ferulic acid concentrations in water-soluble extract of rosemary with satisfactory results.