Western blot法检测CIN宫颈液基细胞残液中HPV16、18E6感染的临床意义

Clinical Significance of Human Papillomavirus(HPV) 16,18E6 Infection in Cervical Liquid-based Cytology Residual Cells from Patients with Cervical Intraepithelial Neoplasis by Western Blot

目的:探讨免疫印记法(Western blot)检测子宫颈上皮内瘤变(cervical intraepithelial neoplasis,CIN)宫颈液基细胞残液中人乳头状瘤病毒(human papillomaviruses,HPV)16、18E6感染的临床意义。方法:采用Western blot法检测经病理证实的86例CIN和14例慢性宫颈炎的宫颈液基细胞残液中HPV16、18E6的感染状况。结果:宫颈液基细胞残液中HPV16、18E6感染的阳性率分别为CINⅠ组33.3%(8/24);CINⅡ组46.7%(14/30);CINⅢ组59.3%(19/32)。慢性宫颈炎(对照组)阳性率7.1%(1/14)。CINⅠ组与对照组比较差异无显著性(P>0.05),CINⅡ组和CINⅢ组与对照组比较均有统计学差异(P<0.05)。结论:Westernblot法检测CIN患者宫颈液基细胞残液中HPV16、18E6感染可以补充细胞学检查结果,对预防宫颈癌发生及降低其死亡率有重要意义。

Objective：To investigate the clinical significance of human papillonmvirus（HPV）16, 18E6 infection in cervical liquid-based cytology residual cells from patients with cervical intraepithelial neoplasis（CIN） by Western blot . Methods： Western blot was used to measure the presence of HPV16, 18E6 infection in cervical liquid-based cytology, residual cells from 86 cases of cervical intraepithelial neoplasis（CIN） and 14 chronic cervicitis, which were confirmed by pathological diagnosis. Results： qhe positive rate of HPV16, 18E6 was 33.3% （8/24） in CINⅠ , 46.7 % （14/30） in CINⅡ , 59.3 % （19/32） in CINⅢ and 7.1% （ 1 / 14 ） in chronic cervicitis （ control group）. The positive rates of HPV16, 18E6 in CINⅡ and CINⅢ were significantly greater than that in control group（ P 〈 0.05 ）, while there was not significant difference between CIN Ⅰ and control group（ P 〉 0.05）. Conclusion： Using Western blot to detect HPV16, 18E6 infection in cervical liquid-based cytology residual cells from patients with cervical intraepithelial neoplasis（ CIN ） can append results to cytologic examnination. It was significant to prevent occurance of cervical carcinoma and decrease its death rate.