固定化微生物对土壤中多环芳烃的降解

Degradation of Polycyclic Aromatic Hydrocarbons in Soils by Immobilized Microorganisms

采用化学方法对微生物进行固定化包埋,通过对3株菌的单独固定及混合固定后来降解有机污染土壤中的芘及苯并(α)芘.实验结果显示,微生物经过固定后对污染物的降解效果明显好于游离菌,同时混合固定菌的降解效率普遍高于单菌固定的降解效率,在96h时芽孢杆菌(Bacillussp.)2号和动胶杆菌(Zoogloeasp.)9号混合固定菌降解效果相对较高,对芘和苯并(α)芘的降解率为61.8%和34.9%.通过不同的采样时间还可以看出,芘的降解速率要快于苯并(α)芘,这是因为芘的苯环数量少.

Microorganisms were immobilized by chemical embedding technique, Three strains were immobilized both separately and jointly to degrade pyrene and benzo（α）pyrene in soil, The results showed that the degradation effect on soil contaminants by immobilized microorganisms was better than that by free ones, and the degradation efficiency of immobilized strain-mixture is higher than immobilized single strain. After immobilized for 96 h the strain-（2-9 ）-mixture provided a high degradation effect, i.e., the pyrene and benzo（α） pyrene were degraded at rate of 61.8% and of 34.9%, respectively. Sampling at different times showed that degradation of pyrene was faster than that of benzo（α） pyrene, since pyrene has less benzene rings. The results as above can offer a certain theoretical basis for the application of immobilized microorganisms in bioremediation of soils contaminated with organic compounds.