用原位杂交和RNA斑点杂交法检测人肝细胞癌和癌旁肝中甲胎蛋白mRNA

In Situ Hybridization and RNA Dot Blot Analysis of Alpha-fetoprotein Messenger RNA in Human Hepatocellular Carcinoma and Its Host Liver

用生物素标记AFP cDNA探针作原位杂交,检测了12例人肝细胞癌和癌旁肝冰冻切片和石蜡组织切片中的AFP mRNA,同时分别抽提了3例人肝癌和癌旁肝总RNA作斑点杂交.结果提示癌和癌旁肝组织中均有AFP基因的过量表达,AFP mRNA在癌内分布比较均一,而在癌旁肝组织中则比较多样,可散在分布、小灶性分布或成片状分布,从而证实不仅肝癌细胞可以自己合成AFP,而且癌旁肝细胞也可以自己合成AFP。

The expression of the alpha-fetoprotein in 12 cases of human hepatocellular carcinoma and its host liver by in situ hybridization analysis of AFP mRNA in cryostat and paraffin - embeded tissue sections was studied. Total RNA was extracted in 3 cases of cancer tissues and its host liver tissues for RNA spot blot Both the tumor cells and the surrounding host liver cells showed positive hybridization with a biotinylated AFP cDNA probe. AFP mRNA existed in almost all tumor cells, but the distribution pattern of AFP mRNA in the host liver varied. The positive cells were dispersed in the tissue or formed foci or appeared to be a small piece of positive area. Our results showed that not only the tumor cells can synthesize AFP, but also the surrounding liver cells have the capacity to produce AFP.