P物质对刀豆素A和美洲商陆诱导小鼠脾细胞产生γ干扰素的调节作用

Regulative Action of Substance P on Interferon-γ Production in Mouse Spleen Cells Induced by Concanavalin A and Pokeweed Mitogen

本实验研究了不同浓度(10^(-11) ～10^(-5) mol/L)P物质(SP)在体外对刀豆素A(ConA)和美洲商陆(PWM)诱导小鼠脾细胞产生γ干扰素(IFN-γ)的影响。结果显示,10^(-11)～10^(-6)mol/L SP对10μg/mlPwM诱生IFN-γ没有影响。用5、2.5及1.25μg/ml ConA或PWM诱生时,10^(-9) ～10^(-5) mol/L SP可增高IFN-γ效价,10^(-7) ～10^(-5)mol/L SP作用更明显。单独加SP而不加诱生剂,未检测到IFN-γ.先加入10^(-9) ～10^(-6) mol/L SP 6h后,再加ConA诱生的IFN-γ效价,略高于同时加入10^(-9) ～10^(-6)mol/L SP时ConA诱生的IFN-γ效价。先加入10^(-5)mol/L SP,IFN-γ效价并不比同时加10^(-5) mol/L SP时增高,反而有降低的趋势.在先加入SP时,10^(-8)mol/L SP即表现出明显的增高IFN-γ效价的作用,而同时加SP,则在10^(-7)mol/L才有明显作用。

The influences of different concentrations (10-11-105mol/L) ofsubstance P(SP)on the interferon -γ(IFN-γ) production in mouse spleen cells induced by concanavalin A (ConA) and pokeweed mitogen (PWM) in vitro were studied. The results showed that no influence of 10-11-10-6 mol/L SP on the titre of IFN-γ induced by PWM (10μg/ml) was observed; the titres of IFN-γ induced by ConA or PWM (5, 2 5 and 1. 25 μg/ ml) were enhanced by 10-9-10-5 mol/L SP; this enhancement was more significant at 10-7-10-5 mol/L SP. No titre of IFN-γ was detected in the presence of SP alone. The titres of IFN-γ induced by ConA added at 6h after adding 10-9-10-6 mol/L SP were slightly higher than that induced by ConA and 10-9-10-6 mol/L SP added simultaneously. The titre of IFN-γ induced by ConA and 10'5 mol/L SP added in advance tended to reduction rather than enhancement as compared with that induced by ConA and 10-5 mol/L added simultaneously. The effect of enhancing IFN-γ production began to appear significantly at lower concentration (10-8 mol/L) of SP added in advance, whereas at 10-7 mol/L SP added simultaneously.