分泌抗ALD-A单克隆抗体杂交瘤细胞株的建立与初步应用

Establishment of the Hybridomas Secreting Monoclonal Antibodies against Rabbit ALD-A and Its Primary Application

用兔肌ALD-A免疫BALB/c小鼠,经融合、筛选后得到5株分泌高效价特异性抗ALD-A单克隆抗体的杂交瘤细胞株,其中E_10株所分泌的单克隆抗体经鉴定属IgM。免疫组化定位研究发现,该抗体除对肌肉组织有弱阳性反应外,对正常人肝、脾、肺、胃和结肠组织均呈阴性反应。而对人肝癌组织则不论是血清AFP阳性者或血清AFP阴性者均呈阳性反应。提示抗ALD-A单克隆抗体在肝癌,特别是AFP阴性肝癌的诊断中,具有潜在的应用前景。

The ALD-A from rabbit muscle was employed to immunize BALB/c mice. After hybridation and selection, five hybridomas secreting high titer anti-ALD-A monoclonal antibodies were prepared, among which the antibody from hybridoma E10 was found to be IgM. It was also found that this antibody did not reacted with normal human liver, spleen, lung, stomach and colon tissues but reacted weakly with normal human muscle. On the other hand, the human hcpatocellular carcinoma tissues were stained by PAP whate-ver the sera of these patients were AFP positive or negative. The results suggest that monoclonal antibodies directed against ALD-A might have potential application in diagnosis of hepatocellular carcinoma especially those with serum AFP negative.