摘要
以芥蓝Brassica alboglabraBailey品种‘尖叶夏芥兰’带叶柄子叶和不带叶柄子叶切块为外植体,探讨了直接分化形成不定芽以及不定芽生根的适宜培养基组成.结果表明:带叶柄子叶比不带叶柄子叶切块的不定芽分化率高,其中在MS+0.05 mg/L NAA+2.0 mg/L 6-BA+30.00 g/L蔗糖+8.00 g/L琼脂(pH 5.8)培养基上,带叶柄子叶的不定芽分化率达93.75%;将分化形成的不定芽转接到不添加NAA和添加0.1 mg/L NAA的生根培养基中,不定芽生根率分别达到100%和86.11%,其中以后者所形成的根多且较粗壮.
Effects of hormone on shoot formation and plant regeneration were studied in reproduction of cotyledon as explant in Brassica alboglabra Bailey. The results showed that shoot formation frequency from the cotyledons with petiole was higher than that of the cotyledon segments without petiole. The maximal shoots formation frequency was 93.75%, When cultured on MS medium containing 0. 05 mg/L NAA, 2. 0 mg/L 6-BA, 30. 00 g/L sucrose and 8.00 g/L agar （ pH 5.8）. The elongated shoots rooted in MS medium without hormone or MS medium containing 0. 1 mg/L NAA, with 100% and 86. 11% of rooting rates, respectively. More and stronger roots were induced in the latter medium.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2006年第4期113-114,共2页
Journal of South China Agricultural University
基金
广东省科技计划项目(2001A2020202)
关键词
芥蓝
子叶
离体培养
植株再生
Brassica alboglabra
cotyledon
in vitro culture
plant regeneration
