摘要
目的分析一株鲍曼不动杆菌耐碳青霉烯类抗生素的机制。方法用三维试验检测碳青霉烯酶,用聚合酶链反应(PCR)扩增和产物测序方法确认耐碳青霉烯酶类抗生素的机制;采用微量肉汤稀释法测定头孢哌酮/舒巴坦和左旋氧氟沙星的MIC值,应用棋盘格局法检测头孢哌酮/舒巴坦和左旋氧氟沙星的联合抗菌效应,计算FIC指数。结果三维试验显示该菌株产碳青霉烯酶,经分子生物学确认有blaOXA-23基因;头孢哌酮/舒巴坦的MIC值为64μg/ml,左旋氧氟沙星的MIC值为32μg/ml,两者联合使用后其MIC都下降一个梯度,计算得FIC=1。结论该菌株产OXA-23碳青霉烯酶,头孢哌酮/舒巴坦与左旋氧氟沙星联合使用后,表现为相加作用。
Object To analyze the cause of Acinetobacter banmamii resisting to carbapenem agent and detect the combinated effect of cefoperazone/sulbactam and levofloxacin. Methods Three-dimension and PCR methods were used to analyze the of mechanism of resisting to carbapenem; The combination effects were deetected by antibiotic susceptibility tests. Resuits blaOXA-23 gene was confirmed in Acinetobacter banmamii; antibiotic sensitive tests showed that cefoperazone/sulbactam＇s MIC was 64 μg/ml and levofloxacin＇s MIC was 32 μg/m. The FIC equal 1 after using both agensts. Conclusion Acinetobacter banmamii can produce OXA-23 carbapenase. There is add effect after combination treatment with cefoperazone/sulbactam and levofloxacin.
出处
《江西医学检验》
2006年第5期406-408,共3页
Jiangxi Journal of Medical Laboratory Sciences
关键词
三维试验
OXA-23基因
碳青霉烯类
鲍曼不动杆菌
联合药敏
Three-dimensional test
OXA-23 gene
Cefoperazone/sulbactam
Levofloxacin
Acinetobacter banmamii
Combination of antibiotic susceptibility test
