钛酸钾生物薄膜/钛合金生物复合材料的制备及其体外生物相容性

Fabrication and biocompatibility in vitro of potassium titanate biological thin film/titanium alloy biological composite

用仿生化学方法制备钛酸钾生物薄膜/Ti-15Mo-3Nb生物复合材料,然后通过模拟体液培养试验、动态凝血试验及体外细胞培养试验对Ti-15Mo-3Nb和钛酸钾生物薄膜/Ti-15Mo-3Nb生物复合材料的体外生物相容性进行研究,以验证钛酸钾作为一种新型的生物活性涂层材料的可行性。对比试验的结果表明:(1)在模拟体液培养实验中,Ti-15Mo-3Nb表面未见钙磷沉积,表现为生物惰性,而呈多孔网状结构的钛酸钾生物薄膜具有很强的钙磷吸附能力,表现出很好的生物活性;(2)以动态凝血时间为指标,钛酸钾生物薄膜>Ti-15Mo-3Nb,表现出良好的血液相容性;(3)细胞培养实验表明,二者均具有良好的细胞相容性,但在细胞培养初期钛酸钾生物薄膜具有更好的细胞附壁生长趋势,这将有利于损伤部位的早期愈合。钛酸钾生物薄膜/Ti-15Mo-3Nb生物复合材料表现出更好的生物相容性和生物活性。

Using the way of bionic chemistry,potassium titanate biological thin film/Titanium alloy was fabricated out. The biocompatibility in vitro of Ti-15Mo-3Nb and potassium titanate biological thin film/Titanium alloy was studied using the simulated body fluid cultivation, kinetic clotting of blood and osteoblast cell cultivation experiments in vitro. By comparing the biological properties of the both materials,we can obtain the conclusion as follows： （1）The deposition of a calaum phosphate layer was not found on the surface of Ti-15Mo-3Nb,so it was bioinert. While the networks of potassium titanate biological thin film could induce the deposition of a calaum phosphate layer, which showed its excellent bioactivity; （2）According to the values of kinetic clotting,the blood coagulation time of potassiun titanate biological thin film was more than that of Ti-15Mo-3Nb. It was obvious that potassiun titanate biological thin film possessed a good hemocompatibility; （3）The cell compatibility of both materials was very well. But growth trend and multiplication of osteoblast cells on the surface of potassiun titanate biological thin film was more excellent, which made for the concrescence of wound during the earlier period. As a result,potassium titanate biological thin film/Titanium alloy showed more excellent biocompatibility and bioactivity.