一氧化氮对体外培养脊髓及背根神经节细胞的作用

Effect of NO on cultured spinal cord and DRG cells

目的：研究一氧化氮（ＮＯ）对体外培养脊髓及背根神经节细胞的作用．方法：应用ＭＴＴ微量比色法观察ＮＯ研究工具药Ｌ┐精氨酸（Ｌ┐Ａｒｇ）和Ｌ┐Ｎ位硝基精氨酸（Ｎ┐Ａｒｇ）对脊髓及背根神经节细胞体外存活和突起生长的影响．结果：一氧化氮合酶（ＮＯＳ）底物Ｌ┐Ａｒｇ（５００、１０００μｍｏｌ／Ｌ）明显抑制体外培养脊髓及背根神经节细胞的存活和突起生长，降低其活性，而ＮＯＳ抑制剂Ｎ┐Ａｒｇ（２００μｍｏｌ／Ｌ）则表现出相反的作用，并可逆转Ｌ┐Ａｒｇ的作用．结论：ＮＯ抑制脊髓及背根神经节细胞的体外存活和突起生长，ＮＯＳ抑制剂Ｎ┐Ａｒｇ则有一定的细胞保护作用．

Objective: To observe the effect of NO on primary cultured spinal cord (SC) and DRG cells from the mice. Method: MTT method. Results: The OD values of L Arginine group (500 μmol/L、 1 mmol/L) were smaller than that of the control group, the OD values of the Nω nitro L Arginine (200 μmol/L) group were larger versus that of control. The latter effect could be reversed by co application of L Arginine (1 mmol/L). Conclusion: NO inhibits neuronal survival and neurite growth of the SC and DRG in vitro , and Nω nitro L Arginine inhibiting NOS has neuroprotective effect.