转EPSPS基因抗草苷膦油菜基因漂移的初步研究

Preliminary Study on the EPSPS Gene from Glyphosate Resistant Brassica napus Move to Wild Brassica napus and Orychophragmus violaceus

The study used glyphosate resistant Brassica napus,planted in the field of Mianyang Normal College.The glyphosate resistant Brassica napus seeds wereplanted in the middle field,and the wild Brassica napus and Orychophragmus Violaceus were planted around the glyphosate resistant Brassica napus,in different directions in nature environment in 25 meters.The second year the wild Brassica napus and Orychophragmus Violaceus seeds were plantde in the field,and sprayed more glyphosate on the plants,and selected the plants which were not died.The leaves of not died wild Brassica napus were checked in the lab by PCR and Southern blot.The result indicated that four wild Brassica napus plant got the EPSPS gene from the glyphosate resistant Brassica napus,there are no gene flow between Orychophragmus Violaceus and glyphosate resistant Brassica napus.

The study used glyphosate resistant Brassica napus, planted in the field of Mianyang Normal College. The glyphosate resistant Brassica napus seeds wereplanted in the middle field , and the wild Brassica napus and Orychophragmus Violaceus were planted around the glyphosate resistant Brassica napus, in different directions in nature environment in 25 meters. The second year the wild Brassica napus and Orychophragmus Violaceus seeds were plantde in the field , and sprayed more glyphosate on the plants , and selected the plants which were not died . The leaves of not died wild Brassica napus were checked in the lab by PCR and Southern blot. The result indicated that four wild Brassica napus plant got the EPSPS gene from the glyphosate resistant Brassica napus, there are no gene flow between Orychophragrnus Violaceus and glyphosate resistant Brassica napus.