人脐血细胞静脉输注对血管性痴呆大鼠行为和海马细胞凋亡的影响

The effect of intravenous administration of human umbilical cord blood cells on behavior and apoptosis in hippocampi in vascular dementia rats

目的观察人脐血细胞(HUCBCs)静脉输注对血管性痴呆(VaD)大鼠行为改善的作用及对海马细胞凋亡的影响。方法大鼠随机分为模型组、治疗组、对照组,各组大鼠又分为2周、4周、8周3个时相点。以穿梭箱对大鼠行为学进行检测。用TUNEL法对大鼠海马凋亡细胞进行检测。结果穿梭箱结果显示2周时模型组大鼠主动回避反应(AAR)比例为(57.6±8.4)%而治疗组为(68.9±7.0)%(P<0.01);4周时模型组为(42.0±9.5)%,治疗组为(69.6±8.2)%(P<0.01);8周时模型组为(43.6±7.5)%,治疗组为(70.8±7.4)%(P<0.01)。治疗组大鼠术后各时相点海马细胞凋亡比例显著低于模型组(P<0.01)。模型组大鼠2周、4周、8周时海马凋亡细胞比例分别为(14.99±2.56)%,(9.73±0.80)%和(5.55±1.02)%。与治疗组的(4.46±1.04)%,(3.66±0.83)%和(3.37±0.86)%比较差异有非常显著性(P<0.01)。结论采用HUCBCs治疗对VaD大鼠的行为有一定的改善作用,对海马细胞有明显保护作用。

Objective To investigate the effect of intravenous administration of human umbilical cord blood cells（HUCBCs） on active avoidance response （AAR） ratio on vascular dementia rats and investigate the effect of these cells on apoptosis of hippocampi in vascular dementia rats. Methods The rats were randomly divided into a model group, a treatment group and a control group. The three groups were observed at 2, 4 and 8 weeks, respectively. Serum samples of rats were taken at 2, 4 and 8 weeks after operation. A widespread nuclear terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling （TUNEL） was used to identify apoptotic cells in hippocampi of the rats. Results Behavioral tests showed the AAR ratio in the treatment group [ 2nd week （68.9 ±7.0）% ,4th week（69.6 ± 8.2）% ,Sth week （70.8± 7.4）% 1 was significant higher than that in the model group. TUNEL positive cells in hippocampus in the control group were obviously more than those in the treatment group. Conclusion Behavioral test showed that HUCBCs therapy could improve cognition function of VaD rats,and intravenous administration HUCBCs could reduce apoptosis cells in hippocampus.