摘要
硫酸铵分级沉淀结合多种层析技术,从螺旋藻(SP-Dz)中纯化到电泳纯别藻蓝蛋白(Allophycocyanin,APC),纯度(A650/A280)达4·83。APC在30min内的荧光扫描曲线为直线;30min连续光照其相对荧光强度仍为原来的98%以上,其抗荧光淬灭能力强于同样条件下的藻蓝蛋白(PC)及荧光素TRITC。用悬滴气相扩散法培养获得了APC晶体。
APC of electrophoresis-purity was obtained from Spirulina using ammonium sulfate grade precipitation followed with several gel chromatography, and the purity （A650/A280） of which attained to 4.83. The fluorescence scan curve of APC was a direct line in 30 min; After exposed in light for 30 min, the relative fluorescence strength of APC preserved above 98 percent of that as begin. APC has a strouger anti-fluorescence quenching than PC or TRITC under the same condition. APC crystals were grown by hanging drop vapor diffusion method.
出处
《微生物学通报》
CAS
CSCD
北大核心
2006年第5期31-34,共4页
Microbiology China
基金
辽宁省教育厅项目(No.20040331)
沈阳农业大学青年教师基金(No.200401)
关键词
钝顶螺旋藻
别藻蓝蛋白
光谱
晶体
Spirulina platensis, Allophycocyanin, Spectrum, Crystal
