药用真菌β-(1,3)-D-葡聚糖构效关系及检测方法研究进展

Progresses in the Studies of Structure-activity Correlation and Detection Methods of Medicinal Fungal β-(1,3)-D-glucans

β-(1,3)-D-葡聚糖是许多活性真菌多糖的核心结构,由于具有免疫调节、抗肿瘤等多种生物活性,已成为研究热点。本文从β-(1,3)-D-葡聚糖的主链、支链分支度、支链基团、分子量及空间结构等方面介绍了结构与生物活性之间的关系,并对目前该类多糖的定性方法及鲎G因子、半乳糖神经酰胺ELISA等定量检测方法进行了综述。

β-（ 1, 3） -D-glucan is a core structure of many bioactive fungal polysaccharides. It has drawn intensive attentions in recent because of its multiple bioactivities, particularly the immunomodulatory and antitumorous actions. In this review, we introduced the studies of structure-activity correlation of these β-（ 1, 3 ） -D-glucans, particularly the contributions of main backbone, branch degree and residues, molecular weight and conformation to the activities. We also summarized the recent progress in the detective methods for both quantitation and qualification using limulus G factor assay, galactosyl ceramide ELISA and etc.