中药抗氧化成分及抗氧化活性的体外评价方法

Antioxidants of Chinese Materia Medica and in vitro Methods for Assessment of Antioxidant Activities

研究发现有机体的多种疾病都与自由基对机体的氧化损伤有关,中药作为天然抗氧化剂资源倍受关注。综述了中药中酚类、黄酮类、皂苷及多糖等几类主要天然抗氧化剂及其抗氧机理,并简要介绍了评价清除自由基作用的几种体外方法,包括化学发光法、分光光度法和电子自旋共振法等。

It has been confirmed that a variety of diseases are associated with reactive oxygen species＇ oxidative damages to biomolecules. Rich in natural antioxidants, Chinese materia medica （CMM） has attracted much attention. The article reviews the main natural antioxidants （phenolics, flavonoids, saponins, polysaccharides and so on） from CMM and their antioxidative mechanisms. And several in vitro methods for assessing antioxidant activities are introduced, including chemiluminescence, spectrophotometic assay and electron spin resonance （ESR） spectroscopy.