聚合酶链反应检测慢性前列腺炎患者前列腺液中沙眼衣原体

Detection of Chlamydia Trachomatis DNA in Prostatic Secretion of Chronic Prostatitis Using PCR

采用聚合酶链反应（ＰＣＲ）对１３４例慢性前列腺炎病人的前列腺液进行沙眼衣原体ＤＮＡ重复序列检测，结果阳性率为２９．１％（３９／１３４）。非慢性前列腺炎其他泌尿生殖道疾病患者的前列腺液１５例作为对照，均未出现阳性反应。用ＰＣＲ检测沙眼衣原体敏感性高（检测３．８５ｐｇＤＮＡ，在引物扩增片段５１７ｂｐ仍有可见的扩增信号），特异性强（与数种细菌、病毒、支原体、弓形虫均未出现沙眼衣原体待异性沉淀带），提示本法可用于临床诊断，在一定程度上替代培养技术，成为监测沙眼衣原体感染的实验室手段。

The repetitive DNA sequences of Chlamydia trachomatis in prostatic secretion of chronic prostatitis was detected by using polymerase chain reaction (PCR). In 134 patients, 39 cases showed positive reaction (29.1%). All of 15 patients with other urogenital diseases but no significant inflammation ,which were selected to be the control,gave negative results. Because of high sensitivity and specificity,the PCR technique can replace the culture techinique,and becomes a major method for the diagnosis of Chlamydia.