肾综合征出血热病毒抗原在肝内胆管上皮的定位

Presence of virus antigens in the epithelial cells of the intrahepatic bile ducts in autopsy cases of hemorrhagic fever with renal syndrome

目的：确定肾综合征出血热（ＨＦＲＳ）病毒抗原在尸检肝组织肝内胆管上皮细胞的定位．方法：免疫组织化学多重ＰＡＰ法．结果：１８例尸检肝组织中，４例肝内胆管发现ＨＦＲＳ病毒抗原，主要分布于肝内大胆管，小叶间胆管和小胆管的上皮细胞中．抗原阳性物质呈细颗粒状，定位于胆管上皮细胞核上区的胞质中．结论：陈旧尸检肝组织肝内胆管上皮细胞胞浆中存在ＨＦＲＳ病毒抗原．可能随胆汁排出在粪便中成为污染来源．

Objective: To determine the presence of hemorrhagic fever with renal syndrome (HFRS) virus antigens in the epithelial cells of the intrahepatic bile duct of autopsy cases of HFRS. Methods: Multiple repeated PAP immunohistochemical method was applied. Results: Among 18 autopsy cases of the HFRS, 4 cases showed HFRS virus antigens in the epithelial cells of the intrahepatic bile duct system, particularly,in the large bile ducts,septal bile ducts and bile ductules. The antigen immunoreactivity was finely granular and located in the supranuclear cytoplasm of bile duct epithelial cells. Conclusion: There are HFRS virus antigens exist in the epithelial cells of intrahepatic bile ducts in autopsy cases of HFRS, which indicates that discharge of virus antigen in the stool is possible.