摘要
采用逆转录病毒载体将hGM-CSF基因导入人膀胱癌细胞株BIU-87细胞中,建立转基因细胞株BIU/GM。经PCR-SouthernBlot杂交证实GM-CSF基因已整合到BIU/GM细胞基因组中。经流式细胞仪细胞DNA周期分析表明,转基因前后BIU-87细胞增殖状态无变化。免疫荧光检测发现GM-CSF基因导入及表达不能促进BIU-87细胞表面HLA-ABC、DR、DQ抗原的表达。转基因细胞经60Gy/sX线灭活后,丧失增殖能力,但能维持一定水平的GM-CSF分泌活性达2周。本文为进行转基因瘤苗的深入研究奠定了初步基础。
The cDNA of hGM CSF was transfered into the human bladder cancer line BIU 87 with retroviral vector. PCR southern blot demonstrated the integration of transfered gene in the BIU cell genome. Biological activity of the hGM CSF secreted by the BIU cell was confirmed. DNA analysis by flowcytomerty showed no influence on growth of the BIU cells. Flourenscent immunoassay revealed no induction of HLA ABC, DR, DQ expression on the surface of the gene transfered BIU cells. The gene transfected BIU cells were irradiated with 6000 rad X ray but they could secret hGM CSF for at least 2 weeks, although the growth of them was abrogated. These suggested that we could develope the bladder cancer vaccine secreting hGM CSF for clinical trials in future.
出处
《中国医科大学学报》
CAS
CSCD
1996年第6期582-585,共4页
Journal of China Medical University
关键词
转基因
膀胱癌
肿瘤疫苗
HGM-CSF
hGM CSF
retroviral vector
gene transfected bladder tumour vaccine