摘要
对103例浙江汉族人载脂蛋白(Apo)B基因XbaⅠ酶切位点,应用限制性片段长度多态性(RFLPs)研究表明:(1)冠心病组中少见X2等位基因的相对频率为0.11,显著高于正常对照组的0.02(P<0.01);(2)X2等位基因的相对频率与冠心病的病变范围无明显关联;(3)冠心病组中具X1X2基因型者与具X1X1基因型者比较,HDL-C及SOD水平明显降低(P分别<0.01,<0.05)。本研究结果表明,ApoB基因XbaⅠ酶切点的RFLPs可能与冠心病的发病有一定关系。
XbaIrestrictionfragmentlengthpolymorphisms(RFLPs)oftheapolipoproteinB(ApoB)genewasexaminedin43unrelatedChinesepatientswithdocumentedcoronaryarterydisease(CAD)and60unrelatedhealthyChinesesubjects,usinggenomichybridizationanalysiswitha5.0kbApoBcDNAprobe.Alelefrequencieswerecomparedbetweenpatientsandcontrols,andtheirimpactonlipidandoxygenfreeradicalsmetabolismwerealsostudied.TherarealeleoftheXbaIpolymor-phism,theX2alele,wasmorefrequentinCADpatientscomparedwithcontrols(0.11vs0.02,P<0.01).CADpatientswiththegenotypeX1X2hadlowerlevelsofHDL-CandSODcomparedtothosewiththegenotypeX1X1(P<0.01,P<0.05,respectively).ThesedatasuggestthatgeneticvariationatXbaIsiteofApoBgenemaycontributetothedevelopmentofCADinChineseindividuals.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
1996年第6期341-344,共4页
Chinese Journal of Medical Genetics
关键词
载脂蛋白
B基因
多态性
冠心病
ApolipoproteinBgeneCoronaryarterydiseaseRestrictionfragmentlengthpolymorphisms