慢性阻塞性肺疾病患者肺内一氧化氮合成酶及其mRNA的变化

Changes of Nitric Oxide Synthase and its mRNA Expression in Lungs of Patients with Chronic Obstructive Pulmonary Disease

以酶组织化学、分子杂交及放免法观察了慢性阻塞性肺疾病（ＣＯＰＤ）患者和对照者肺组织中一氧化氮合成酶（ＮＯＳ）的分布、ｍＲＮＡ表达及活性。结果表明：ＮＯＳ广泛分布于对照组支气管、肺泡管和部分肺泡囊上皮细胞及肺血管内皮；ＣＯＰＤ组支气管上皮细胞及肺小动脉内皮ＮＯＳ活性较对照组低，但在肺间质和肺血管周围出现ＮＯＳ阳性细胞增多；斑点和Ｎｏｒｔｈｅｒｎ印迹杂交显示ＣＯＰＤ组肺组织ＮＯS基因ｍＲＮＡ水平较对照组明显低；ＣＯＰＤ组肺组织和肺小动脉环磷酸鸟苷含量均较对照组明显降低（Ｐ＜０．０１）。提示一氧化氮在肺脏中发挥重要生理作用及在ＣＯＰＤ发病中可能具有重要意义。

sing NADPH-diaphorase histochemical method,Northern blot analysis,and radioim-munoassay,we have studied the distribution and activity as well as mRNA expression of nitric ox-ide synthase(NOS)in lungs of 8 patients with chronic obstructive pulmonary disease(COPD)and 6 without COPD. The data showed that 1.NOS is present in epithelial cells of bronchi,alveo-lar duc,and alveolar sac;and in endothelium,but rare in media and adventitia of pulmonary ves-sels in patients with or without COPD. However,the activity of NOS markedly decreased in pa-tients with COPD as compared with that in those without COPD,2.The mRNA expression ofNOS in patients with COPD(0.34±0.11U)was significantly lower than that in those withoutCOPD(0.84 ± 0.19U)(P<0.01)3.cGMP level of lung tissues in patients with or withoutCOPD was43.8±5.2 and 86.6±7.5pmol/g wet wt.respectively(P<0.01),and that of pul-monary small arteries in patients with or without COPD was 56.8± 6.4 and 89.6±8. 6pmol/gwet wt.respectively(P<0.01).These results indicate that nitric oxide may play an importantrole in physiology of lung and pathogenesis of COPD.