颞下颌关节骨关节病髁突软骨细胞雌激素受体β差异表达的研究

Study on Differential Estrogen Receptor β Expression of Mandibular Condylar Chondrocyte in Temporomandibular Joint Osteoarthritis

目的探讨大鼠颞下颌关节骨关节病髁突软骨细胞雌激素受体(βERβ)的差异表达。方法25只SD大鼠随机分为实验组(15只)和对照组(10只)。实验组以去除左侧部分关节盘的方法建立大鼠颞下颌关节骨关节病模型,术后3个月处死动物,取其髁突软骨细胞进行培养,采用免疫组化、逆转录聚合酶链反应、肽质量指纹谱(PMF)和Westernblot等方法观察软骨细胞中ERβ蛋白和mRNA的表达。结果PMF显示,ERβ是软骨细胞线粒体蛋白质组的成分,Westernblot、逆转录聚合酶链反应和免疫组化结果显示,ERβ在实验组细胞和线粒体中表达水平较对照组降低(P<0.01)。结论ERβ可能通过线粒体途径参与了颞下颌关节骨关节病的病理过程。

Objective To test the differential Estrogen Receptor （ER） beta levels of mitochondrial proteome of mandibular condylar chondrocyte in the rat model of temporomandibular joints osteoarthritis （TMJOA）. Methods 25 SD rats were divided into experimental group （15 rats） and control group （10 rats） randomly. TMJOA models were created in left sides of TMJ of 15 SD rats by the partial resection of the articular disc. The experimental rats were killed 3 months after operation. After the chondrocytes culture, Immunohistochemistry, semiquantitative RT-PCR and Western blot were used to test the differential ER beta expression levels in mitochondrial proteome of mandibular condylar chondrocytes. Mitochondrial proteins identification was carried out by two-dimensional electrophoresis and peptide mass fingerprint（PMF）. Results PMF showed that one of the differential proteins was ERβ. Immunohistochemistry and Western blot results suggested the significant difference of ERβ protein levels between operation-treated and control group. The operation-treated group had lower ERβ levels（P〈0.01）. Conclusion It has been demonstrated that ERβ protein levels were decreased in mitochondria of TMJOA mandibular condylar chondrocytes, which suggests a role for mitochondrial ERβ in the effects on TMJOA. The pathological role of ERβ in the regulation of TMJOA progress remains to be elucidated.