白细胞介素4在异位性皮炎中的变化

THE CHANGES OF INTERLEUKIN-4 IN THE PATIENTS WITH ATOPIC DERMATITIS

目的：探讨ＩＬ－４在异位性皮炎发病机制中的意义。方法：采用ＥＬＩＳＡ试剂盒测定异位性皮炎（ＡＤ）、非典型性异位性皮炎（ＡＡＤ）患者和正常对照组ＰＨＡ刺激外周血单一核细胞（ＰＢＭＣ）培养上清液中ＩＬ－４水平，应用免疫组化技术观察ＡＤ皮损浸润单一核细胞中ＩＬ－４和ＩＦＮ－ｒ表达。结果：ＡＤ组ＰＢＭＣ培养上清液中ＩＬ－４水平显著高于ＡＡＤ组或正常对照组，并且ＩＬ－４与ＩｇＥ呈正相关。结论：异位性皮炎患者血清ＩｇＥ和ＩＬ－４呈正相关；异位性皮炎和非典型性异位性皮炎或对照组的免疫病理的不同，支持ＩＬ－４可以作为异位性皮炎的重要诊断指标。

Objective:To investigate the significance of IL-4 in the immunopathogenesis of atopic der-matitis(AD).Methods:IL-4 level,in the supernatants of PBMC of patients with Atopic derrmatitis(AD)and Atypical form of AD(AAD)induced by PHA,was measured by ELISA Kit.Expression of IL-4 and IFN-γ in the mononuclear cells of the skin lesions of patients with AD was studied by immunohisto-chemical staining method.Results:IL-4 level in the supernatants of PBMC of patients with AD was significantly higher than that of patients with AAD and that of control.IL-4 level was related to serum lgE level in patients with AD,and IL-4 positive mononuclear cells in dermis were detected(6/8),but not IFN-γ(0/8).Conclusion:(1)Elevated serum lgE in patients with AD may be related to ele-vatedIL-4.(2)Difference of immunology and immunopathogenesis between AD and AAD or control suggested that IL-4 can be considered as a diagnostic indicator for AD.