复混肥料中化肥含量对3种肥料微生物存活率的影响

Influence of chemical fertilizer content in compound fertilizer on survival rates of three species of fertilizer microbes

将圆褐固氮菌Azotobacter chroococcumAccc 8011,巨大芽孢杆菌Bacillus megatheriumAccc10011和胶质芽孢杆菌Bacillus mucilaginosusAccc 10013的草炭吸附制剂直接或间接(将菌剂装入聚丙烯薄膜袋密封)置于有机肥与化肥制成的复混肥料中,于25℃恒温放置30 d,采用标准平板菌落计数法测定活细菌数量,研究添加化肥对细菌存活率的影响。实验结果表明,当化肥总养分(N+P2O5+K2O)达到0.066 g.g-1,加入的细菌活菌数显著下降。经方差分析,各处理间差异极显著。将这几种细菌的草炭吸附制剂封装入聚丙烯薄膜袋再埋入化肥与有机肥制成的复混肥料中,当化肥总养分达0.122 g.g-1时,细菌的存活率也有显著的下降。所以这3种肥料细菌不能与化肥质量分数较高的有机-无机复混肥料混合,而应单独包装,分开放置。

After putting the preparation of Azotobacter chroococcum Accc 8011, Bacillus megathrium Accc 1001 and B. mucilaginosus Accc 10013 directly or indirectly （sealing up the preparation in the polypropylene membrane bag） in the organic-inorganic compound fertilizer at 25 ℃ constant temperature for 30 days, bacterium＇s survival rate was determined with the standard colony counting method. The result indicated that the survival rate of the bacterium evidently reduced when content of chemical fertilizer in the organic-inorganic compound fertilizer reached 0.066 g·g^-1 （N ＋ P2O5 ＋ K2O total weight）, but the survival rate of the bacterium in the bag evidently reduced when content of chemical fertilizer in the organic-inorganic compound fertilizer reached 0.122 g·g^-1. Therefore, these three kinds of fertilizer bacterium preparations couldn＇t be mixed with organic-inorganic compound fertilizer with high content of chemical fertilizer. They should be separately wrapped up and placed.