反相高效液相色谱法测定复方小檗片中盐酸小檗碱的含量

Determination of Berberine Contained in Prescription of Berberine Pill by RP-HPLC

目的 采用反相高效液相色谱（RP-HPLC）法测定复方小檗片中盐酸小檗碱的含量。方法 用Kromasil色谱柱（51μm，250mm×4．6mm），流动相：乙腈-水-磷酸二氢钾-十二烷基磺酸钠（500ml：500ml：3．0g：1．5g），流速：1ml·min^-1，柱温：40℃，检测波长为345nm。结果 在0．0212-0.2332μg之间，峰面积与进样量的线性关系为Y=3513 092．99X-15 938．76（r=0．9999），平均回收率为97．22％。测得3批片剂中盐酸小檗碱的含量分别为0．772，0．748，0．749mg／片。结论 所建方法简便、准确、重复性好，可用于复方小檗片中盐酸小檗碱的含量测定。

Objective To establish an RP-HPLC method to determine berberine from prescription of berberine. Methods The column were Kromasil （5μn,250 mm×4.6 ram） ,the mobile phase consisted of methyl cyanide：water： KDP： SDS（500 ml： 500 ml： 3.0mg： 1.5mg） ,the flow rate was 1 ml/min and the UV detector was set at 345 nm. Results The method displayed good linearity within the concentration ranges of 0. 021 2 - 0.233 2μg（ r =0.999 9）. The average recovery was 97.22%. The contents of three lots of prescription of berberine were 0.772, 0. 748,0.749 mg/pill. Conclusion This method is simple, accurate, replicate and suitable for the determination of berberine in prescription of berberine.