高效液相色谱法测定复方厚朴颗粒中厚朴酚及和厚朴酚的含量

Determination of Magnolol and Honokiol in Compound Houpu Granule By HPLC

目的 建立HPLC法测定复方厚朴颗粒中厚朴酚及和厚朴酚的含量。方法 采用Diamondsil^CM C18色谱柱（6．4mm×200mim，5μm）；流动相为甲醇-0．1％磷酸溶液（78：22）；流速1ml／min；柱温35％℃；检测波长294nm。结果 厚朴酚的线性范围为0．0411—0．8222μg，r=0．9999，平均回收率为97．1％，RSD=1．27％；和厚朴酚的线性范围为0．02306—0．4612μg，r=1，平均回收率为96．7％，RSD=1．25％。结论 该方法结果准确，重现性好，可作为复方厚朴颗粒质量控制的定量方法。

Objective To establish an HPLC method for the determination of magnolol and honokiol in Compound Houpu Granule. Methods The samples were analyzed on a Diamondsil^CM C18 column （6.4 mm × 200 mm,5μm）. The mobile phase was a mixture of MeOH -0.1% H3PO4 （78：22） ;the rate of flow was lml/min; the eolunm temperature was 35℃ ; the detection wavelength was 294 nm. Results Calibration graph was linear in the range of 0. 041 1-0. 822 2 μg, r = 0. 999 9 for magnolol, 0.023 06 -0.456 12 μg, r = 1 for honokiol. The recovery was 97.1%, RSD = 1.27% for magnolol; 96.7%, RSD = 1.25% for honokiol. Conclusion The method is sensitive and its results are accurate with good reproducibility. It can be used for the quality control of Compound Houpu Granule.