摘要
以三聚氯氰为活化剂制备硫脲修饰戊二醛交联壳聚糖衍生物,用红外光谱和元素分析法对产物进行了初步表征,并研究了硫脲修饰戊二醛交联壳聚糖对牛血清白蛋白(BSA)的吸附及洗脱性能。硫脲修饰交联壳聚糖对牛血清白蛋白的吸附容量达596 mg.g-1(干),其吸附行为符合Freundlich和Langmuir等温吸附方程。硫脲修饰交联壳聚糖装柱(Φ=1.0cm×10cm)吸附BSA后,分别以1.0 mol.L-1 NaCl和0.2 mol.L-1(NH4)2SO4溶液洗脱,牛血清白蛋白的洗脱效率分别为98.2%和96.4%。硫脲修饰交联壳聚糖制备工艺简单,吸附容量较大且容易洗脱,可用作蛋白质分离纯化的吸附剂。
The preparation of thiourea modified glutaraldehyde-crosslinked chitosan resin activated with cyanuric chloride, and its adsorptivity to bovine serum albumin （BSA） was studied. The successful modification of thiourea to chitosan chains was proved with infrared spectra and element analysis. The maximum adsorption capacity of thiourea modified chitosan resin to bovine serum albumin was 596 mg ·g^-1 （dry resin）. The adsorption was correlated with Freundlich and Langmuir isotherm equation, respectively. The BSA adsorbed on the column （φ= 1.0 cm×10 cm） of thiourea modified chitosan resin can be eluted with 50 mL of 1.0 mol·L^-1 NaCl and 0.2 mol·L^-1 （NH4）2SO4 solution with the recovery of 98.2% and 96.4%, respectively. The thiourea modified crosslinked chitosan resin can be applied to the separation and purification of proteins.
出处
《安徽工业大学学报（自然科学版）》
CAS
2006年第4期399-403,共5页
Journal of Anhui University of Technology（Natural Science）
基金
安徽省高校自然科学基金资助课题(2004kj066)
关键词
壳聚糖
硫脲
三聚氯氰
牛血清白蛋白
吸附
chitosan
thiourea
cyanuric chloride
bovine serum albumin
adsorption
