黄淮地区大豆花叶病毒株系的鉴定与分布

Identification and Distribution of SMV Strains in Huang-Huai Valleys

【目的】鉴定黄淮地区大豆花叶病毒株系,明确该地区目前SMV株系的组成、分布和流行情况。【方法】2001～2002年采集了黄淮地区四省28个县市的大豆病样591份,经初步繁殖鉴定、生物纯化及组织印迹检测,得到了50个SMV毒株,采用王修强等筛选的10个鉴别寄主进行接种鉴定。【结果】检测到SC-3～SC-9等7个株系群,发现1个新的株系群SC-10。综合本单位1998～2002年的结果,黄淮地区(河南、山东、安徽北、江苏北)在SC-1～SC-10中,除SC-2未发现外,9个株系群中,以SC-3和SC-7为主,分别占29.21%和23.60%,SC-4和SC-8其次,占10.11%和8.99%。按省分布,河南省有7个株系群,SC-3、SC-4为主,SC-5、SC-7其次;山东省4个株系群,以SC-3为主,SC-8也占相当比重;皖北7个株系群,以SC-7和SC-9为主,其次SC-10;苏北8个株系群,以SC-3和SC-7为主,其次SC-8。按株系群,SC-3、SC-4各省均有;SC-5、SC-6、SC-7在河南、皖北、苏北3地;SC-1、SC-8在河南、山东、苏北3地;SC-9只在皖北发现;SC-10在皖北、苏北2地发现;SC-2在黄淮未发现。【结论】在黄淮地区检测到7个株系群SC-3～SC-9,发现了1个新株系群SC-10。各株系群在黄淮地区的分布不同,各省分中株系组成上也有较大差别。

[Objective] To identify SMV strains in the Huang-Huai Valleys and to reveal their distribution, local constitution, and relative importance in the area. [Method] In 2001 - 2002, 591 diseased specimens of soybean leaves collected from the Huang-Huai Valleys were purified, identified through direct tissue blotting immunoassay （DTBIA）, and classified with their response to ten differential hosts proposed by Wang et al. [ Result] Fifty SMV isolates were acquired. From them, seven SMV strain groups, SC-3 - SC-9, were identified and a new strain group SC-10 was discovered. The analysis of the data in present study （2001 - 2002） combined with those from Wang and Yang （1998 - 2000） showed that in Huang-Huai Valleys （Henan, Shandong, north Anhui and north Jiangsu） nine strain groups among SC-1 - SC-10, except SC-2, existed with the major ones being SC-3 and SC-7 accounting for 29.21% and 23.60%, respectively, and second ones being SC-4 and SC-8 accounting for 10.11% and 8.99%, respectively. In Henan there were seven strain groups with SC-3, SC-4 as major ones and SC-5, SC-7as second ones; in Shandong, four ones with SC-3 as major one and SC-8 as second one; in north Anhui, seven ones with SC-7 and SC-9 as major ones and SC-10 as second one; in north Jiangsu, eight ones with SC-3 and SC-7 as major ones and SC-8 as second one. As for the individual strain groups, SC-3 and SC-4 existed in all the four provinces, SC-5, SC-6 and SC-7 in Henan, north Anhui and north Jiangsu, SC-1 and SC-8 in Henan, Shandong and north Jiangsu, SC-9 only in north Anhui, SC-10 only in north Anhui and north Jiangsu, while SC-2 was not found in Huang-Huai Valleys. [Conclusion] Seven strains, SC-3 - SC-9, were identified and a new strain group SC-10 was discovered. Both different distribution in the area among strain groups and different strain constitution among provinces were explored.