人甲状旁腺素(1-34)对新生Wistar大鼠心肌成纤维细胞增殖及胶原合成的影响

The Effects of Parathyroid Hormone on Proliferation and Collagen Synthesis of Rat Cardiac Fibroblasts

目的:研究外源性人甲状旁腺素(1-34)hPTH(1-34)对原代培养新生Wistar鼠心肌成纤维细胞(CFs)增殖及胶原合成的影响。方法:分离、培养Wistar乳鼠心肌成纤维细胞,加入不同浓度的hPTH(1-34)共培养,四氮唑盐比色法(MTT)、3H-TdR掺入法检测细胞增殖;3H-Proline掺入法测定胶原合成。结果:随着一定浓度hPTH(1-34)的升高,CFs MTT法A490值及3H-TdR的掺入量、3H-脯氨酸掺入率呈明显的递增趋势(P<0.01);维拉帕米预处理组与单独应用组10-8mol/L hPTH相比,以上指标均显著降低(P<0.01)。结论:PTH可显著刺激成纤维细胞增殖及胶原合成,L型钙通道开放引起的细胞外钙内流增加为其重要机制之一。

Objective：To investigate the effects of parathyroid hormone （PTH） on proliferation and collagen synthesis of rat cardiac fibroblasts （CFs）, Methods：Isolated and cultured CFs of Wistar rats were cultured with PTH. CFs proliferation was measured by MTT assay, The DNA synthesis of CFs was measured by 3 H - TdR uptake test. Collagen synthesis was measured by 3 H - Proline incorporation. Results：A490 value of CFs by MTT assay , CFs proliferation and the synthesis of collagen increased graduately with the elevation of hPTH concentrations was enhanced remarkably by constantly action of different concentration of hPTH（1-34） incubation（P〈 0.01）; Compared to the group in which 10^-8mol/L hPTH was used separatdy, group pretreated with 10^-5mol/L verepamilshowed a significant decrease in index above（P〈 0.01 ）. Conclusion： PTH dose - dependently increased DNA and collagen synthesis of CFs, partly due to augmented entry of calcium into call via voltage - dependent Ca^2 ＋ channel.