特发性肾病综合征患儿外周血单个核细胞泛素的表达及意义

Expression of Ubiquitin in Peripherial Blood Mononuclear Cells of Children with Idiopathic Nephrotic Syndrome

目的:探讨泛素的表达与特发性肾病综合征发生、发展的关系。方法:应用实时荧光定量PCR法测定不同治疗效应特发性肾病综合征患儿外周血单个核细胞泛素的mRNA水平。结果:初发特发性肾病综合征(idiopathicnephrotic syndrome,I NS)组泛素mRNA的表达明显低于正常对照组;未用泼尼松等治疗药物之前激素部分效应组的泛素mRNA表达和激素敏感组之间无明显差别;治疗后激素部分效应组和激素敏感组泛素mRNA的表达均较治疗前明显降低,但两组之间无明显差别。结论:泛素表达降低,参与了特发性肾病综合征的发生。泼尼松等免疫抑制剂可提高泛素的表达,逆转泛素低表达引起的免疫紊乱而起到治疗作用。

Objective：Observe the relationship between expression of ubiquitin and the onset or development of idiopathic nephrotic syndrome（INS） in children, Methods：We observed mRNA level of ubiquitin in peripherial blood mononuclear cells of children with idiopathic nephrotic syndrome by the method of real - time quantitative PCR, Results： The ubiquitin mRNA level in INS group was significantly lower than that in normal control before treatment, After the treatment, the ubiquitin mRNA level in INS group increased significantly. The ubiquitin mRNA level in children with prednisone - sensitive INS was not significantly higher than that of children with partial prednisone - sensitive INS before and after treatment. Conclusion：The expression of ubiquitin might be linked with onset of INS. Prednisone and other immune inhibitory drugs might be efficient on the treatment of INS partially through improving the level of ubiquitin in peripherial blood mononuclear cells.