HPLC法测定氯沙坦钾胶囊含量

Determination of losartan potassium capsules by HPLC

目的建立高效液相色谱法测定氯沙坦钾胶囊含量，为质量控制提供有效的分析手段。方法色谱柱：Phenomene×ODSC18（5μm、4．6mm×150mm）；流动相：乙腈 -0．1％磷酸溶液（45：55）；检测波长：230nm。结果制剂中辅料和有关物质对主药无干扰，氯沙坦钾在浓度100．2—350．8mg·L^-1范围内线性关系良好，相关系数r=0．9999；平均回收率分别为99．36％、99．68％、99．40％，RSD为0．42％。结论本法专属性好，准确，简便，可有效控制本品质量。

Aim In order to control the quality of losartan potassium capsules, a HPLC method for the determination of losartan potassium capsules was developed. Methods The chromatographic column of Phenomene × ODS C18 （ 5μm,4.6 mm × 150 mm） was used. The mobile phase consisted of acetonitrile 0. 1% phosphoric acid solution （ 45 ： 55 ）. The detection wavelength was at 230 nm. Result Alfuzosin and the other components in the samples were separated completely. The relationship between response and the concentration on the range of 100.2 - 350.8 mg· L^-1 was linear with the correlation coefficient of 0. 9999. The average recovery were 99.36% ,99.68% and 99.40% respectively. RSD =0.42%. Conclusion The method was specific,accurate and simple.