摘要
目的:建立高效液相色谱法测定格列本脲片的含量。采用KromasilC18色谮柱.流动相为甲醇-乙腈。磷酸二氢铵溶液(pH3.50)(55:15:30).流速为1.0ml·min^-1.检测波长为301.0nm,进样体积为10μl。线性范围为10~300μg·ml^-1(r=0.9999.n=6),高、中、低3种浓度的平均加样回收率为100.3%.RSD%为0.71%(n=9)。本方法简便、准确、专属性强,灵敏度高.内标物易得。
To develop an HPLC method for the determination of Glibenclamide in tablets. The determination was performed on a Kromasil C18 column with the mobile phase consisting of CH3OH-CH3CN-NH4H2PO4 (pH3.50)(55 : 15 : 30). The flow rate was 1.0ml.min^-1. The detection was made at 301.0nm. The injection volume was 10μl. The method offered good linearity in the range of 10-300μg . ml^-1(r= 0. 9999,n = 6). The average recovery was 100. 3^ for three different levels of the added amount of Glibenclamide with RSD= 0. 71^(n= 9). This method was convenient ,accurate,specific and sensitive. The internal standard was easy to obtain.
出处
《中国药品标准》
CAS
2006年第4期39-40,共2页
Drug Standards of China
关键词
格列本脲片
HPLC法
含量测定
Glibenclamide tablets
HPLC tcontent determination